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. 2018 May 11;17(8):1627–1636. doi: 10.1074/mcp.RA118.000767

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© 2018 Karim Malet et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

Author's Choice—Final version free via Creative Commons CC-BY license.

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Fig. 6. — Pore-formation dependent down-regulation of host proteins by LLO and PFO. Immunoblot analysis of HeLa incubated for 30 min with wild-type (WT) or pore-deficient (mut) LLO and PFO toxins. Antibodies against UBE2K (Ubiquitin-conjugating enzyme E2 K), UBE2N (Ubiquitin-conjugating enzyme E2 N) and UBC9 (SUMO-conjugating enzyme UBC9) were used to monitor changes in protein levels. Actin is shown as a loading control. Wild-type toxins were used at similar hemolytic titers. Mutant toxins were used at similar protein concentration as the corresponding wild-type toxins.