Table III. Averaged TMT phosphopeptide abundances (from at least three biological replicates) of the substrate mycelium (MII30h) and sporulating aerial mycelium (MII65h) compared to the MI stage (16 h), of the phosphopeptides and phosphoproteins discussed in the manuscript. The MII/MI ratio is shown in the logarithm (log 2) and linear forms. N.S. Not significant (q-value higher than 0.01). (-) significant abundance values that did not pass the 2-fold threshold. Phosphorylation sites are highlighted in bold.
| Category | PROTEIN | Phosphopeptide | Log 2 (MII30h/MI) | Log 2 (MII65h/MI) | Fold-change (MII30h/MI) | Fold-change (MII65h/MI) |
|---|---|---|---|---|---|---|
| Regulatory | SCO0204 | AHpSEQGDNTGSPVR | n.s. | 3.1 | n.s. | 8.5 |
| DevA (SCO4190) | ALQEDGLLTNVpSK | n.s. | 2.1 | n.s. | 4.2 | |
| DasR (SCO5231) | STDVSSAENEGGApTVR | n.s. | 2.1 | n.s. | 4.3 | |
| SCO5357 | VNASAEQAAPADDAPpSER | n.s. | 1.6 | n.s. | 3 | |
| SCO5544 | GHDEPDpSSRTDR | n.s. | 3.2 | n.s. | 9.2 | |
| SCO5544 | GHDEPDSpSRTDRTPR | n.s. | 1.9 | n.s. | 3.7 | |
| SCO5544 | GHDEPDpSpSRTDRTPR | n.s. | 3.6 | n.s. | 12 | |
| SCO5704 | IDIRPDTEQPSDASPEQSpSGGRGE | n.s. | 4.8 | n.s. | 27.8 | |
| SCO7463 | TSDTPGASSEGHEVpS | n.s. | 2.8 | n.s. | 6.8 | |
| Cell division | DivIVA (SCO2077) | QLETQADDpSLAPPR | n.s | 1.6 | n.s | 3.1 |
| SepF (SCO2079) | IAEGGFFNQpS | n.s. | 1.1 | n.s. | 2.1 | |
| SCO2082 (FtsZ) | VTVIAAGFDGGQPPpSK | n.s. | 2 | n.s. | 4 | |
| SCO4439 | DGDADGYDGPPPVDQPpTAVFK | 1.3 | 1.9 | 2.4 | 3.7 | |
| Chaperones, thioredoxin and stress | SCO1836a | SGQDTIETpSGpTAK | n.s. | −1 | n.s. | 0.49 |
| PspA (SCO2168a) | QAIEGGQGQGEApSpSQSQQPQDTPR | n.s. | 1.72 | n.s. | 3.3 | |
| GroES (SCO4761) | IVVQPLDAEQpTTASGLVIPDTAK | - | 2.2 | - | 4.5 | |
| GroES (SCO4761) | IVVQPLDAEQTTApSGLVIPDTAK | n.s. | 2.5 | n.s. | 5.5 | |
| GroES (SCO4761) | IVVQPLDAEQTpTASGLVIPDTAK | n.s. | 2.6 | n.s. | 5.9 | |
| SCO5419 | NVLAEEPGNpTEAK | n.s. | 1.6 | n.s. | 3.1 | |
| Protein synthesis (translation) | RspA (SCO1998) | AAAEGVDTAGAAPAASGGGGGGSYSSEGGDNpSGALASDEALAALR | n.s. | 2.1 | n.s. | 4.3 |
| RspA (SCO1998) | AAAEGVDpTAGAAPAASGGGGGGSYSSEGGDNSGALASDEALAALR | n.s. | 2.4 | n.s. | 5.4 | |
| SCO4703 | AVDpTEGSEA | n.s. | 3.3 | n.s. | 9.6 | |
| SCO4711 | S.E.pSNVTEETK | n.s. | 3.3 | n.s. | 10.2 | |
| Catabolism | SCO1646a | ApTRpSpTEEVEEQAQDAQASEDLK | n.s. | 1.1 | n.s. | 2.2 |
| SCO1646 | pSTEEVEEQAQDAQASEDLK | n.s. | 1.2 | n.s. | 2.3 | |
| SCO1646 | ApTRSTEEVEEQAQDAQASEDLK | n.s. | 1.6 | n.s. | 3 | |
| SCO1646a | ApTRpSTEEVEEQAQDAQASEDLK | n.s. | 3.1 | n.s. | 8.5 | |
| SCO5864 | KTDDDVDSDpSLEELK | n.s. | 3.3 | n.s. | 9.9 | |
| SCO5864 | TDDDVDpSDSLEELK | n.s. | 3 | n.s. | 8 | |
| SCO5864 | KTDDDVDpSDSLEELK | n.s. | 4.4 | n.s. | 21.1 | |
| Nucleotide metabolism | PurE (SCO3059) | EFQQDLNDQApTEK | n.s. | 3.2 | n.s. | 9 |
| SCO3542 | ELPQIDPDQAPPpSR | n.s. | 2 | n.s. | 4 | |
| SCO3542 | ELPQIDPDQAPPpSRR | n.s. | 2.2 | n.s. | 4.5 | |
| Folate biosynthesis | SCO3401 | SAPFAQGPSDPTVQPVPASVIEQVDAADTpTLSNPK | n.s. | −1.5 | n.s. | 0.3 |
| Glycoslisis | SCO7443 | TSGLADGVVVTPpSHNPPADGGFK | n.s. | 1.4 | n.s. | 2.7 |
| Unknown | SCO2668 | AGDIDGApTAK | n.s. | 1.1 | n.s. | 2.1 |
| SCO2668 | VEEADEMTLETRpSpTKpTVR | n.s. | 1.9 | n.s. | 3.8 | |
| SCO2668 | AVQLAGVSGNADpTAK | 1.62 | 1.3 | 3.1 | 2.5 | |
| SCO3859 | SQGGSVLSNTTpTTTpSpSSGAPTVK | n.s. | 3 | n.s. | 8.2 | |
| SCO3859 | SQGGSVLSNTTTpTTpSpSSGAPTVK | n.s. | 3.4 | n.s. | 10.4 | |
| SCO3859 | SQGGSVLSNTTTpTpTpSpSSGAPTVK | n.s. | 3.3 | n.s. | 9.6 | |
| SCO3859 | SQGGSVLSNTTTTpTpSpSSGAPTVK | n.s. | 3.4 | n.s. | 10.4 | |
| SCO3859 | SQGGSVLSNpTTTTTSpSSGAPTVK | n.s. | 3.2 | n.s. | 9.3 | |
| SCO3859 | SQGGpSVLSNTTTTTSpSSGAPTVK | n.s. | 3.5 | n.s. | 11.2 | |
| SCO3859 | SQGGSVLSNTTTTTpSpSSGAPTVK | n.s. | 3.9 | n.s. | 14.8 | |
| SCO3859 | SQGGSVLSNTTTTpTSpSSGAPTVK | n.s. | 5.3 | n.s. | 40.2 | |
| SCO3859 | SQGGSVLSNTpTTTTSpSSGAPTVK | n.s. | 5.9 | n.s. | 61.1 | |
| SCO3859 | SQGGSVLSNTTTTpTSpSpSGAPTVK | n.s. | 3.4 | n.s. | 10.9 | |
| SCO3859 | SQGGSVLSNTTpTpTTpSpSSGAPTVK | n.s. | 3 | n.s. | 8 | |
| SCO3859 | SQGGSVLSNpTpTpTTTSpSSGAPTVK | n.s. | 4.1 | n.s. | 17 |
aOnly one phosphorylation site, at one of the positions marked, exists. See supplemental Table S2.