Figure 6. Demethylation and reexpression of SUSD2 in adipocytes.
(A). qRT-PCR validation of genes significantly hypomethylated and upregulated in Methyl-Capture- and RNA-seq data from RNA derived from same adipocytes as Fig. 5 (performed in duplicate). (B) qRT-PCR validation of the same genes from RNA derived by 3 different adipocyte samples (performed in triplicate) (*P < 0.05, **P < 0.01). (C). Integrated Genome Browser view of SUSD2 gene promoter region, CpG islands, methylation, and RNA-seq data (performed in duplicate).