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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Cancer Res. 2018 Jun 11;78(15):4370–4385. doi: 10.1158/0008-5472.CAN-17-3993

Figure 2. Paclitaxel resistance is associated with increased stem-like properties in ovarian cancer MCTS.

Figure 2

(A) Graphical representation of 2D and 3D culture growth for paclitaxel treatment. (B) Ovarian MCTS showed a robust decrease in paclitaxel sensitivity compared to 2D cultures. (C) Immunofluorescence detection of Ki-67, and DAPI, reveals a decrease in Ki-67 expression following paclitaxel treatment in MCTS. (D) Gene expression was increased following 1 µM paclitaxel treatment in MCTS compared to 2D cultures measured using qRT-PCR (n=3) (*= p< 0.05, t-test). (E) Graphic representation of spheroid disassociation experiments. (F) Cells grown in 2D culture derived from vehicle (blue) or paclitaxel (red) pretreated spheroids have reduced paclitaxel response compared to standard 2D cultured cells (green). (G) Transcript expression of stem-like genes is maintained in 2D cultures derived from vehicle (blue) or paclitaxel (red) pretreated spheroids for three days. All data are represented as mean +/− SD, n=3.