Figure 4. Licofelone and glafenine decrease stemness in ovarian cancer MCTS.

(A-B) Dosage response to the top two 3D specific drug hits, licofelone and glafeline, following 72 h treatments shows both drugs are more sensitive in ovarian MCTS than 2D cultures. (C) Expression of stem-like transcripts following 72 h, IC₅₀ dosage treatment of both drugs in MCTS was measured using qRT-PCR. (D) Immunofluorescence detection of Ki-67 and DAPI reveals an increase in Ki-67 expression following treatment with licofelone or glafeline at their respective IC₅₀ values in MCTS. (E) Correlation of baseline licofelone expression in 2D and 3D to either the IC₇₅ of licofelone (top panel) or the IC₅₀ of licofelone (bottom panel). All data are represented as mean +/− SD, n=3.