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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Mol Cancer Ther. 2018 May 10;17(8):1694–1704. doi: 10.1158/1535-7163.MCT-18-0028

Figure 2. Cellular TOP1cc induced by the fluoroindenoisoquinolines in human cancer cells.

Figure 2

A, TOP1cc detected by ICE bioassay. Human leukemia CCRF-CEM and colon carcinoma HCT116 cells were treated with the indicated drugs (1 μM) for 1 h at 37°C. DNA-containing fractions were blotted and TOP1 was detected using TOP1 C21 monoclonal antibody. B, Quantitative analysis of TOP1cc detected as DNA-protein complexes (DPC) by alkaline elution. Human leukemia CCRF-CEM cells were treated for 1 h at 37°C as indicated.