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. 2018 Aug 2;8:11606. doi: 10.1038/s41598-018-29707-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antibiotic susceptibility testing of 49 clinical isolates using (a) porB, and (b) rpmB as RNA AST markers. Antibiotic susceptibility of 49 clinical isolates (9 susceptible and 40 resistant) from the Neisseria gonorrhoeae panel of the CDC Antimicrobial Resistance Isolate Bank was determined using the “normalized” C:T ratios (C:T ratio of marker/C:T ratio of 16S rRNA). Clinical isolates were exposed to ciprofloxacin for 10 min and the concentration of RNA markers was measured by digital PCR.