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. 2018 Aug 2;8:11613. doi: 10.1038/s41598-018-30037-0

Figure 3.

Figure 3

Phylogenetic tree of nucleotide sequences of TCRβ V segments of bank vole and mouse. The tree was inferred using the neighbour-joining method. Bootstrap values greater than 50, based on 1000 replicates, are shown. Branch lengths are in units of evolutionary distances (the number of base substitutions per site). The full list of mouse reference sequences with accession numbers is available in Supplementary Table S1. Sequences of bank vole genes identified in this study are available in Supplementary File S3; only one allele for each locus is present herein for clarity. Orange circles mark the mouse sequences (TRBVXX*01); red circles and tree branches represent murine pseudogenes (P) or sequences in an open reading frame but with alterations in regulatory elements or splice sites (ORF); and blue circles mark the bank vole sequences (BV_TRBVXX*01). Number after asterisk refer to the allele name.