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. 2018 Aug 2;8:11613. doi: 10.1038/s41598-018-30037-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mean V–J segment usage and CDR3 length distribution of TCRβ in seven bank voles. (a) Relative usage frequencies of each BV_TRBV gene. (b) Weighted mean (by the number of reads) of the CDR3 amino acid length for each BV_TRBV gene. (c) Relative usage frequencies of each BV_TRBJ gene. (d) Weighted mean (by the number of reads) of CDR3 amino acid length for each BV_TRBJ gene. (e) CDR3 amino acid length distribution. In (a,c,e): the bar height indicates the respective mean frequency from seven individuals, error bars show standard deviations. In (b,d): Tuckey boxplots represent weighted mean CDR3 length formed by each contributing gene segment in seven individuals; whiskers indicate data points within the 1.5 interquartile range; the dashed line marks the global CDR3 length median.