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. 2018 Aug 2;8:11585. doi: 10.1038/s41598-018-30025-4

Figure 1.

Figure 1

Establishment of FMRP-deficient human NPC lines. (A) Representative immunofluorescence staining of SSEA-4 (green) in TIG-WT and TIG-KO cells. Nuclei (blue) were stained with DAPI. Scale bars represent 100 μm. (B) Relative FMR1 mRNA levels were determined using qRT-PCR analysis. The expression of FMR1 was normalized to that of GAPDH in each cell clone. The FMR1 mRNA level in TIG-WT cells was arbitrarily assigned as 1. The data are presented as mean ± S.D. of three independent experiments. N.D.: not detected. (C) Schematic illustration of differentiation conditions for NPC from iPSC. (D) Representative immunofluorescence staining of the expression of SOX2 (red) and NESTIN (green) in iNPC-WT and iNPC-KO lines. Scale bars represent 100 μm. (E) Western blot analysis of FMRP protein levels in iNPC-WT and iNPC-KO lines. GAPDH was used as a loading control. The representative images in replicated experiments (n = 2) were cropped and shown in this figure. Whole gel images are presented in Supplementary Fig. S1D.