Figure 5.

Functional characterisation of neural spheroids derived from the FMRP-deficient NPCs. (A) Schematic illustration of the NS formation from iNPCs. Medium with LX7101 or DMSO was changed every three days. (B) Representative immunofluorescence staining of MAP2 (gray) and VGLUT1 (red) in iNS-WT and iNS-KO lines. Nuclei (blue) were stained with DAPI. Scale bars represent 20 μm. (C) Representative immunofluorescence staining of MAP2 (red) in iNS-WT and iNS-KO lines. Nuclei (blue) were stained with DAPI. Scale bars represent 100 μm. (D) The rates of cells that exhibited at least one firing during the observation period. The data are presented as mean ± S.D. of three independent experiments (*p < 0.05 relative to iNS-WT).