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. 2018 Aug 2;8:11585. doi: 10.1038/s41598-018-30025-4

Figure 6.

Figure 6

LX7101 affects protein phosphorylation in the AKT-mTOR pathway. (A) Western blot analysis of AKT signalling pathways in ReN-WT, DMSO-treated and LX7101-treated ReN-KO lines. The representative images in replicated experiments (n = 2) were cropped and shown in this figure. Whole gel images are presented in Supplementary Fig. S5A. (B) Western blot analysis of AKT signalling pathways in iNPC-WT, DMSO-treated and LX7101-treated iNPC-KO lines. The representative images in replicated experiments (n = 2) were cropped and shown in this figure. Whole gel images are presented in Supplementary Fig. S5B. (C) Western blot analysis of p-AKT (S473) and pan-AKT protein levels in ReN-WT, DMSO-treated and MK-2206-treated iNPC-KO lines. GAPDH was used as a loading control. The representative images in replicated experiments (n = 2) were cropped and shown in this figure. Whole gel images are presented in Supplementary Fig. S5C. (D) Quantification of GFAP area/cell in iNPCs 72 h after treatment with DMSO or MK-2206. The intensity of GFAP in DMSO treated iNPC-KO was arbitrarily assigned as 1. Error bars represent ± S.D. of three independent experiments (***p < 0.001).