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. 2018 Aug 2;8:11631. doi: 10.1038/s41598-018-30100-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Correlation of allelic frequency of ctDNA and CA19-9 levels with patient response to therapy. The allelic frequencies of (a) M_P/KRAS, (b) M_P/FNA, and (c) M_P/TR were box-plotted based on evaluations of their near-time response to therapy and diagnosis. (d) CA 19-9 levels were box-plotted. All of the determined levels are presented on a logarithmic scale. In each box-plot, median and mean are indicated by a horizontal bar and red diamond, respectively. The level of statistical significance (ANOVA, LSD) is indicated as *P ≤ 0.05, **P ≤ 0.001, and ***P ≤ 0.00001. CR, complete response; Dx, diagnosis; PD, progressive disease; PR, partial response; SD, stable disease.