Figure 5.

Knockdown of C1GalT1 blocks the radiation-enhanced invasiveness in esophageal cancer cells. Cells were irradiated at 0-4 Gy and grown for 24 h before the following experiments. (A) The scratch wound healing assay was performed in ECa109 cells. Data are expressed as the percentage of wound closure compared with the unirradiated cells (0 Gy). (B) Transwell invasion assay was performed to assess the invasive ability of ECa109 cells. Data are represented as ratios versus unirradiated cells (0 Gy). (C) Comparison of the migratory abilities of ECa109-NC and ECa109-si cells with or without 4 Gy irradiation. (D)Comparison of the invasion abilities of cells with or without 4 Gy irradiation. All the images were acquired at 100× magnification. *P<0.05, **P<0.001.