Figure 1.

Establishment of the acquired cisplatin-resistant T24 cells.

Notes: (A) Cell viability of UBC cells treated with cisplatin at various concentrations (0–16 μg/mL) for 72 h detected by MTT assay. Data are presented as mean ± SE. (B) Western blotting analysis on UBC cells for the expression levels of NFκB/RelA transcriptional factor, which were normalized by actin as a loading control. (C and D) The quantification analysis of Western blotting data. The band density was quantified by Image J software and normalized values were depicted in graphs.

Abbreviations: UBC, urinary bladder cancer; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; SE, standard error; NFκB, nuclear factor kappaB; RQ, relative quantification.