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. 2018 Jul 30;11:4413–4429. doi: 10.2147/OTT.S162255

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© 2018 Xue et al. This work is published and licensed by Dove Medical Press Limited

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Zyflamend had cytotoxic effect and downregulated the NFκB signaling pathway in UBC cells.

Notes: (A) Flow cytometry analysis on T24R and J82 cells treated with Zyflamend (50 μg/mL) and cisplatin (1 μg/mL) alone or both using an Annexin V-FITC/PI double staining kit. Representative plots are shown. (B and C) Quantification of cell death rates in T24R (B) and J82 cells (C) treated with Zyflamend and cisplatin alone or both. Annexin V-positive cells were defined as apoptotic cells. Data are presented as mean ± SE; *P<0.05; **P<0.01. (D and E) Western blotting analysis on T24R (D) and J82 cells (E) treated with Zyflamend and cisplatin alone or both for 48 h. FL1-H is for Annexin V and FL2-H is for PI.

Abbreviations: NFκB, nuclear factor kappaB; UBC, urinary bladder cancer; T24R, cisplatin-resistant T24 cell; PI, propidium iodide; SE, standard error; FITC, fluorescein isothiocyanate.

Zyflamend had cytotoxic effect and downregulated the NFκB signaling pathway in UBC cells.

Notes: (A) Flow cytometry analysis on T24R and J82 cells treated with Zyflamend (50 μg/mL) and cisplatin (1 μg/mL) alone or both using an Annexin V-FITC/PI double staining kit. Representative plots are shown. (B and C) Quantification of cell death rates in T24R (B) and J82 cells (C) treated with Zyflamend and cisplatin alone or both. Annexin V-positive cells were defined as apoptotic cells. Data are presented as mean ± SE; *P<0.05; **P<0.01. (D and E) Western blotting analysis on T24R (D) and J82 cells (E) treated with Zyflamend and cisplatin alone or both for 48 h. FL1-H is for Annexin V and FL2-H is for PI.

Abbreviations: NFκB, nuclear factor kappaB; UBC, urinary bladder cancer; T24R, cisplatin-resistant T24 cell; PI, propidium iodide; SE, standard error; FITC, fluorescein isothiocyanate.