| Cell cultivation |
Culture dishes |
High cell densities and continuous downstream processing |
| Cell harvesting |
Centrifugation |
Centrifugation or filtration cell suspension |
| Cell disruption |
Three cycles of freeze/thaw |
Microfluidization |
| Dislodge cellular debris |
Centrifugation and filtration |
Centrifugation and tangential flow filtration |
| Primary separation |
No |
Anion-exchange chromatography |
| Secondary separation |
Ultracentrifugation on a CsCl gradient |
Chromatography: anion-exchange, affinity, immobilized metal affinity, hydrophobic interaction, size exclusion |
| Desalination |
Desalination column or dialysis, ultrafiltration |
Desalination column or dialysis, ultrafiltration |
