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. 2018 Jun 7;19(8):e46182. doi: 10.15252/embr.201846182

Figure EV3. Effects of cGAS knockdown with or without concomitant mtDNA depletion in DENV‐infected DCs.

Figure EV3

  • A–C
    Human DCs were transfected with control siRNA (Ctl) or three sets of cGAS siRNA duplexes for 24 h and then infected with mock or DENV (MOI = 5) for an additional 24 h. As indicated, ddC treatment to deplete mtDNA was introduced in some conditions. The cGAS, viral NS3 and β‐actin protein levels (A, n = 4), cGAS mRNA (B, n = 4) and TLR9 mRNA, DENV mRNA, and IFN‐λ1 mRNA (C, n = 4) were determined. Values are means of individual measurements in each sample ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 (one‐way ANOVA multiple comparisons with Bonferroni post hoc test).

Source data are available online for this figure.