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. 2018 Jul 6;19(7):1981. doi: 10.3390/ijms19071981

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Trastuzumab resistance is associated with epithelial-mesenchymal transition (EMT) in gastric cancer cells. (a,b) MKN45/R and NCI N87/R cells showed morphological changes when compared with their parental cells respectively; (c) Images of cells stained with antibodies against β-tubulin (red), and nuclei stained with DAPI (blue) (original magnification ×10 for NCI N87 and NCI N87/R cells, ×20 for MKN45 and MKN45/R cells respectively); (d) Expression of E-cadherin, N-cadherin, vimentin, snail1, and twist in trastuzumab-resistant cells and their parental cells as detected by western blot; (e) NCI N87, NCI N87/R, MKN45, and MKN45/R cells were labeled with antibodies against E-cadherin, N-cadherin, and vimentin. Binding was detected by Alexa Fluor^® 594-labeled secondary antibody. Staining of nuclei was done with 1 μg/mL DAPI.

Trastuzumab resistance is associated with epithelial-mesenchymal transition (EMT) in gastric cancer cells. (a,b) MKN45/R and NCI N87/R cells showed morphological changes when compared with their parental cells respectively; (c) Images of cells stained with antibodies against β-tubulin (red), and nuclei stained with DAPI (blue) (original magnification ×10 for NCI N87 and NCI N87/R cells, ×20 for MKN45 and MKN45/R cells respectively); (d) Expression of E-cadherin, N-cadherin, vimentin, snail1, and twist in trastuzumab-resistant cells and their parental cells as detected by western blot; (e) NCI N87, NCI N87/R, MKN45, and MKN45/R cells were labeled with antibodies against E-cadherin, N-cadherin, and vimentin. Binding was detected by Alexa Fluor^® 594-labeled secondary antibody. Staining of nuclei was done with 1 μg/mL DAPI.