Figure 2.

Trastuzumab resistant cells exhibit aggressive migration. (a,b) The confluent cell monolayers were wounded by scraping and the closure of the wounded areas was monitored. The images of the wounds were taken at different time points; (c,d) Migration activities were analyzed by measuring the distance cells traveled toward the center of the wound; (e,f) Colony formation assays were performed and colony numbers were counted and analyzed. All experiments were performed in triplicate. * p < 0.05, ** p < 0.01 vs. controls.