Figure 4.

Expression of AfSPL14 transcripts in various tissues of A. fasciata and immunoblot analysis of AfSPL14 in central leaves treated with or without ethephon. (a) Expression level of AfSPL14 transcripts in various tissues of juvenile and adult plants. (1) juvenile plants; (2) adult plants prior to flower bud differentiation; (3) 39-DAF flowering adult plants. Samples were collected at 10:00 am. (b) Expression level of AfSPL14 transcripts in the vegetative and reproductive organs of 39-DAF flowering adult plants. Samples were collected at 10:00 am. (c) Expression level of AfSPL14 transcripts in the central leaves of A. fasciata in response to exogenous ethephon treatment at different concentrations for different time. In the panels, 0, 1, 2, 4, and 8 h represents the samples collected at 10:00, 11:00, 12:00, 14:00, and 18:00, respectively; 24 h and 48 h represent the treated samples collected at 10:00 am at the next day and the next two days, respectively. For CK, 10 mL of distilled deionized H₂O was poured into the cylinder shapes of A. fasciata. 0 h represents the samples treated without ethephon or distilled deionized H₂O. (d) Immunoblot analysis of the AfSPL14 protein level in the central leaves of A. fasciata treated with 10 mL of 0.6 g·L⁻¹ exogenous ethephon for 1, 8, and 24 h, or without ethephon (0 h). The total proteins were separated using SDS-PAGE, and the transferred proteins were then probed with a rabbit polyclonal AfSPL14 antibody or a rabbit polyclonal Actin antibody, respectively. (e) Relative level of AfSPL14 protein in the central leaves of A. fasciata treated with 10 mL of 0.6 g·L⁻¹ exogenous ethephon for 1, 8 and 24 h, or without ethephon (0 h). Three independent experiments were performed, the values are shown as the means and error bars indicate the standard deviation (n = 3). ANOVA was conducted, and means were separated by DNMRT.