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. 2018 Jul 18;19(7):2085. doi: 10.3390/ijms19072085

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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RT-qPCR analysis of flowering related genes at the shoot apex of WT, Vector and Pro35S::AfSPL14 transgenic plants. Relative expression of three flowering promoting genes, SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) (a), FRUITFULL (FUL) (b), LEAFY (c), and one florigen Flowering Locus T (FT) (d), and three flowering organ identify genes, APETALA1 (AP1) (e), AP2 (f) and AP3 (g) was performed. Fourteen-day-old long-day-grown seedlings were used. Three biological replicates and three technical replicates were performed. Transcript levels were normalized using AtACTB gene as a reference. All primers used here are listed in Table S3 online. ANOVA was conducted, and means were separated by DNMRT.