Figure 5.

Effects of catalpol on intracellular Ca²⁺ levels, cytosolic LDH activity, cell survival, and maturation of PreOLs under OGD and 2-[2-[4-(4-nitrobenzyloxy)phenyl]ethyl]isothiourea (KB-R7943) treatment. (A) Representative Ca²⁺ response traces under resting condition in the CTL, OGD, CAT, and KB-R7943+catalpol-treated group (KB+CAT) groups. (B) Quantification of intracellular Ca²⁺ levels at the resting condition measured by Fluo-3 staining in the CTL, OGD, CAT, and KB+CAT groups. (C) Quantification of LDH activity in the CTL, OGD, CAT, and KB+CAT groups. (D) Quantification of TUNEL-positive cells in the CTL, OGD, CAT, and KB+CAT groups. (E) Western blot probing for MBP in the CTL, OGD, CAT, and KB+CAT groups. β-actin was used as a loading control. The blot shown is representative of six independent experiments. (F) Quantification of MBP protein expression levels from the Western blot analyses. Data are shown as means ± SEM (n = 6 in each group). * p < 0.05; ** p < 0.01; *** p < 0.001.