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QRT-PCR detection of the temporal expression patterns of the candidate miRNAs. The expressions of 15 miRNAs on rat pituitaries of different ages (Day 7, Day 40, Day 90, and Day 250) were detected by qRT-PCR. The values are expressed as the means ± SEM. Means with different letters were significantly different (n = 8; p < 0.05; ANOVA; Tukey-HSD).
