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. 2018 Jul 15;19(7):2058. doi: 10.3390/ijms19072058

Figure 7.

Figure 7

miR-141-3p negatively regulates growth hormone (GH) mRNA and protein expression. GH3 cells were separately transfected with 100 pM miR-141-3p mimic and miR-141-3p inhibitor. Forty-eight hours later, GH3 cells were collected. (a,b) Analysis of miR-141-3p levels by qRT-PCR. (c,d) Analysis of GH1 mRNA levels by qRT-PCR. (e,f) Analysis of GH protein levels by Western blot and quantitative analysis of the Western blot results. Results are presented as the means ± SEM (n = 3). *, p < 0.05, **, p < 0.01, (t test). iNC, inhibitor negative control.