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. 2018 Jul 27;9:1094. doi: 10.3389/fpls.2018.01094

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Copyright © 2018 Kumar, Tannous, Sionov, Keller and Prusky.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of early (EH) and late harvested (LH) apple fruit on the relative expression of laeA and patulin cluster, patulin accumulation and colonized surface following the inoculation of Penicillium expansum in Golden Delicious fruits. Apple fruits cv. Golden Delicious were harvested first 135 days after fruit set (early harvest) and 156 days after fruit set (late harvest) and inoculated immediately. Five days later the colonization pattern (A), laeA relative expression (B), patulin accumulation in the colonized tissue (C), and the relative expression of the pat cluster genes (PCG) (D) was evaluated. RNA was prepared from the leading edge of the colonized area. Average values (±SE) of five replicates are reported. For statistical analysis, the unpaired student t-test was used with significance defined as a P value (^∗P ≤ 0.05; ^∗∗P ≤ 0.01; ^∗∗∗P ≤ 0.001).