FIGURE 8.

Effect of epicatechin in absence and presence of different sucrose levels on patulin accumulation and laeA relative expression. Solid SM media at initial pH 5.0 were amended with 0, 15, 175 mM sucrose and combined with epicatechin at concentrations ranging from 0 up to 1 mM. The effect of the combination of sucrose/epicatechin was tested on laeA relative expression (RE) (A,C,E) and patulin accumulation (B,D,F). The media was inoculated with 100 μl of a 10⁶ spore/ml suspension. Patulin and laeA relative were evaluated on the third day post-inoculation. Five 10-mm diameter disks were sampled from five independent culture plates. Average values (±SE) of five replicates are reported. Experiments were repeated three times and results of a single representative experiment are shown.