Figure 1.

The total amounts of endothelial differentiation-related factor 1 (EDF1) and peroxisome proliferator-activated receptor gamma (PPARγ) in cells treated with VEGF. Human umbilical vein endothelial cells (HUVEC) were treated with 50 ng/mL of vascular endothelial growth factor (VEGF) for 0, 8, 12, and 24 h. (a) Real-Time PCR was performed on RNA samples. Two different experiments in triplicate were performed; (b) cell lysates were analyzed by western blot using antibodies against EDF1, PPARγ, and actin. A representative blot is shown.