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. 2018 Jun 21;19(7):1830. doi: 10.3390/ijms19071830

Figure 2.

Figure 2

Subcellular localization of EDF1 in cells treated with VEGF. (a) HUVEC were treated with VEGF (50 ng/mL) for 1, 8, 12, and 24 h. Immunofluorescence was performed using anti-EDF1 immunopurified immunoglobulin G (IgGs) and rhodamine-conjugated anti-rabbit IgGs; (b) HUVEC were treated with VEGF (50 ng/mL) for 1 h. Western blot was performed on nuclear and cytosolic fractions using antibodies against EDF1. GAPDH and TBP were used as cytosolic and nuclear markers, respectively. A representative blot is shown.