Figure 3.

The interaction between EDF1 and PPARγ in HUVEC treated with VEGF. HUVEC were treated with VEGF (50 ng/mL) for different times. Cell lysates were immunoprecipitated with monoclonal antibodies against PPARγ and analyzed by western blot using rabbit antibodies against EDF1 (upper panel). The filter was then probed with rabbit anti-PPARγ antibodies to verify the equal amounts of immunoprecipitated proteins (lower panel). Densitometric analysis was performed using ImageJ software. EDF1/PPARγ ratio was calculated on three blots from separate experiments ± standard deviation.