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. 2018 Jun 21;19(7):1830. doi: 10.3390/ijms19071830

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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PPARγ transcriptional activity in HUVEC with silenced EDF1. (a) The modulation of PPARγ was evaluated in αs1 cells (αs1) (HUVEC with stably silenced EDF1) and compared to HUVEC transfected with a scrambled nonsilencing sequence (used as control) (CTR). Cell lysates were analyzed by western blot using antibodies against EDF1, PPARγ, and actin. A representative blot is shown; (b) PPARγ activity was evaluated by luciferase assay in αs1 cells and compared to the control HUVEC; (c) Real-Time PCR was performed on RNA samples from αs1 cells and relative control, treated or not with VEGF (50/ng/mL) for 24 h. Three different experiments in triplicate were performed; (d) Nitric oxide (NO) release was measured using the Griess method for nitrate quantification. The values were expressed as the mean of three different experiments in triplicate ± standard deviation. * p < 0.05, ** p < 0.01, *** p < 0.001.