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. 2018 Jun 19;19(8):e45607. doi: 10.15252/embr.201745607

Figure 6. Interaction of TPX2 with ADD1 and Eg5 is important for spindle pole integrity.

Figure 6

  1. HeLa cells were infected with lentiviruses expressing shRNAs to TPX2 (sh‐TPX2), or luciferase (sh‐Luc.). HA‐TPX2 WT or the mutants (∆1–43, ∆236–370 and ∆711–747) were re‐expressed in the cells whose endogenous TPX2 had been depleted. The cells were stained for γ‐tubulin (green), centrin2 (red), and DNA (blue). The insets are magnification of the centrin2 signal at the indicated poles. Scale bars, 5 μm.
  2. The percentage of multiple γ‐tubulin foci in the total number of mitotic cells was measured (308–1,116 mitotic cells were counted in each group).
  3. The percentage of poles with n centrioles found in cells with multiple γ‐tubulin foci was assessed (746–1,239 poles were counted in each group).
Data information: Values (means ± s.d.) are from three independent experiments. **P < 0.01 and ***P < 0.001 (Student's t‐test). For clarity, not all significances are indicated.