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. 2018 Jun 12;19(8):e45642. doi: 10.15252/embr.201745642

Figure EV4. Treatment with BI at different time points. Related to Fig 5 .

Figure EV4

  1. Mean RGd2 fluorescence levels plotted against the coefficient of variation for cells in 2i, or differentiated in BI or control for different time points.
  2. Percentage of Nanog‐positive and Klf4‐positive cells over the differentiation time‐course in the presence of BI or control. Mean and SD shown; n = 3; unpaired two‐tailed Student's t‐test.
  3. Bulk mRNA expression of naïve pluripotency factors in a differentiation experiment in the presence or absence of BI. Expression is relative to Actb. Mean and SD shown; n = 2.
  4. Percentage of RGd2‐positive cells when BI is added at different intervals, relative to control, DMSO‐treated, cells. Mean and SD shown; n = 3; unpaired two‐tailed Student's t‐test.
  5. Percentage of Sox1‐ and Oct4‐positive cells on day 3 of neural differentiation when BI was added at different intervals. Mean and SD shown; n = 3; unpaired two‐tailed Student's t‐test.