Figure 3.

Inhibitory effects of chrysin on histological alterations (A) and collagen fiber deposition (B) in diabetic glomeruli. Diabetic mice were orally administrated with 10 mg/kg chrysin for 8 weeks. PAS staining was done in glomeruli of chrysin-treated db/db mice and nuclear counter-staining was done with hematoxylin (A). Masson’s trichrome staining were performed for glomerular collagen fibers (B) The collagen fibers were stained in blue and muscle fibers were stained in red. Each photograph is representative of at least four animals. Scale bar = 25 μm. The relative staining intensity was measured and the respective values are expressed as mean ± SEM (n = 4 mice in each group). Values in bar graphs not sharing a same lower case indicate significant different at p < 0.05.