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. 2018 Jul 8;19(7):1995. doi: 10.3390/ijms19071995

Figure 4.

Figure 4

The melatonin attenuated the expression of SHH, GLI1 and MMP-1 related proteins in t-BOOH or UVB treated HaCaT keratinocytes. (a) HaCaT cells were exposed to UVB (30 mJ/cm2) for 10 min or 20 min and subjected to Western blotting for p-ERK, PP38, COX-2, MMP-1 and β-actin. (b), (c) Cells were pretreated with melatonin for 24 h, irradiated with UVB (30 mJ/cm2) for 10 min and then subjected to Western blotting for SHH, GLI1 (nuclear fraction), p-ERK, COX-2, p-NF-κB, MMP-1, β-actin and Lamin B. Cells were pretreated with melatonin for 24 h and then with t-BOOH (0.6 mM) for 6 h. Graphs represent quantitative analysis of protein expression adjusted by β-actin. Data represent means ± SD. *** p < 0.001, ** p < 0.01 and * p < 0.05 versus UVB control, ### p < 0.001, ## p < 0.01 and # p < 0.05 versus normal control.