Table 2.

Summary of yeast-based techniques applicable in studies on proteins involved in neurodegenerative diseases.

Technique	Used for	Description
Split-GFP system [111,112,113,114,115,236]	Protein–protein interaction	GFP fluorescence is reconstituted when its two subunits are in close proximity.
Synthetic genetic array [237]	Synthetic lethality	Approach for the systematic construction of double mutants for large-scale mapping of synthetic genetic interactions.
Yeast two-hybrid [238]	Protein–protein interaction	Protein interaction leads to reporter gene expression.
Prion-forming assay [233]	Prion forming	The prion domain of the yeast Ure2 prion is replaced by a potential prion domain of any protein. Reporter gene expression is induced if this domain can complement for the Ure2 prion domain.
Yeast transcriptional reporting aggregating proteins (yTRAP) [235]	Prion forming	High-throughput quantitative prion forming assay. Uses fluorescence as quantifiable reporter.