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Sympathetic innervation approaches NMJs in various skeletal muscles. Diaphragm muscle was from P30, tibialis anterior, soleus, and levator auris longus muscles were from P90 wildtype mice. Except for diaphragm muscle, which was prepared as a whole mount, fiber bundles were teased from these muscles as indicated and stained for NMJs (BGT, red in overlays) and sympathetic neurons (TH, green in overlays). Images show maximum z-projections of confocal image stacks. Scalebars, 20 µm.
