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. 2018 Jul 12;10(7):888. doi: 10.3390/nu10070888

Figure 5.

Figure 5

(A,B): Effect of Resveratrol and/or 5-FU on NF-κB activation and NF-κB-regulated gene end-products involved in apoptosis, metastasis induced by TNF-β in HCT116 and HCT116R in inflammatory microenvironment. Alginate cultures of HCT116 (A) and HCT116R (B) were treated for 10 days as described in detail in “Section 2”. Immunoblotting with whole cell lysates was performed with antibodies against p65-NF-κB, CXCR4, MMP-9 and cleaved-caspase-3. Western blots shown are representative of three independent experiments. The housekeeping protein β-actin served as a positive loading control in all experiments. (C,D): Effect of resveratrol and/or 5-FU on TNF-β-induced epithelial-to-mesenchymal transition of CRC cells in tumor microenvironment cultures. Colorectal cancer cells in alginate culture were treated as described in Materials and Methods. After 10 days whole cell lysates of HCT116 (C) and HCT116R (D) were subjected to western blotting with antibodies against vimentin, E-cadherin and slug. Western blots shown are representative of three independent experiments. The housekeeping protein β-actin served as a positive loading control in all experiments.