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. 2018 Jul 4;19(7):1958. doi: 10.3390/ijms19071958

Figure 4.

Figure 4

Pro-apoptotic activity of LV50. (A) Morphological analysis of Jurkat nuclei stained with Hoechst 33258 after incubation with 10 μM LV50 for 72 h. Vehicle-treated cells are uniformly stained and the nuclei are intact, indicating that cells are viable; treatment causes nuclear fragmentation and condensation. A representative example of three independent experiments. (B, left panel) Analysis of apoptosis of Jurkat cells (treated with 10 μM LV50 for 4 and 24 h) by biparametric flow cytometry analysis after double staining with fluorescein isothiocyanate (FITC) Annexin V/PI staining. Numbers represent the percentage of Annexin V-single positive cells (early apoptosis, upper left quadrant) or Annexin V/propidium iodide (PI)-double positive cells (late apoptosis, upper right quadrant). (B, right panel) The results shown in bar graphs were obtained from three independent experiments and are reported as mean ± SD. Histograms show the percentages of single Annexin V and Annexin V/PI-double positive cells. ** p < 0.01 versus vehicle.