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. 2018 Jul 23;19(7):2134. doi: 10.3390/ijms19072134

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Scrape-loading dye-transfer assay to measure GJIC ability. The wtCx26, icCx26, and mock clones were soaked in a cocktail of Lucifer yellow CH and RITC-dextran, scraped by a micropipette tip, and observed under a fluorescence microscope after 5 min of incubation. (a) Representative micrographs of 3 different clones. The same fields of each clone were captured. Note that dye-coupled cells with Lucifer yellow CH were observed only in the wtCx26 clone. (b) Histogram showing the mean GJIC capacity of each clone. Error bars represent the SD (n = 6).