Figure 1.

Three isolates of live bacterial species isolated from the gallbladder of a patient with cholecystitis were subjected to 16S rRNA gene sequencing and phylogenetic analysis. The DNA relatedness of sequences with known taxa is shown using the unweighted pair group method and arithmetic average method (UPGMA). The optimal tree with the sum of branch length was 1.04, 0.51, and 1.22 for panels (A–C), respectively. All positions containing gaps and missing data were eliminated, and the analyses were conducted in MEGA7.