Figure 1.
BCAA supplementation conditionally increased body weight in mice fed a low protein diet. Body weight (A), body length (B), and femur length. (C) were measured (n = 12–20 mice/group). The average daily food intake (g/cage/day normalized by the number of mice per cage) and energy intake (kcal/cage/day normalized by the number of mice per cage) (D) and lean and fat mass gain normalized to energy intake (E) were also calculated (n = 6–7 cages/group). Data represent mean ± standard error of the mean (SEM). Statistical analyses of main effects (protein, BCAA, and time) and interactions (protein*BCAA and protein*BCAA*time) are shown below each graph. * p < 0.05 for low protein vs. low protein + BCAA in panel A. BCAA: Branched-chain amino acids.