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. 2018 Jul 14;10(7):903. doi: 10.3390/nu10070903

Figure 4.

Figure 4

A twenty-eight day protocol with five ip injections efficiently triggers an anti-CMP IgE immune response in BALB/c mice. (a) Three protocols of sensitization were evaluated in BALB/c mice (n = 5–6 per group). These protocols differ in the frequency of ip injections and timepoints for blood collection. After the ip injection of different concentrations of CMP (0.025, 0.05 or 0.25 mg) in a final volume of 250 μL, blood samples were collected, and the levels of anti-CMP IgE antibodies evaluated. Different letters mean statistical differences (p < 0.05) by one-way ANOVA and Tukey’s multiple comparison tests. (b) Serum samples from mice (n = 4–6 per group) with low or undetectable anti-CMP IgE levels were screened for the presence of anti-CMP IgG antibodies. Different letters mean statistical differences (p < 0.05) by Kruskall–Wallis test and Dunn’s multiple comparison tests. (c) Groups of mice (n = 5) were injected ip with CMP following either a 14- or 28-day protocol with 2 or 5 ip injections respectively. The presence of anti-CMP IgE antibodies in 14- and 28-day serum samples was evaluated before and after IgG depletion. Different letters mean statistical differences (p < 0.05) by paired t test. All serum samples were evaluated using ELISA.