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. 2018 Jul 2;19(7):1941. doi: 10.3390/ijms19071941

Figure 1.

Figure 1

Cecropin A inhibited bacterial adherence and reduced the expression of inflammatory factors. The porcine jejunum epithelial cells (IPEC-J2) cells were pretreated with cecropin A (3.125, 6.25, and 12.5 μg/mL) for 48 h, and the CFU of adherent bacteria were counted ((A), n = 3). The relative mRNA expression of TNF-α, IL-8, and IL-6 were tested by using qPCR ((B), n = 6). Control: control group; cecropin A: cells were pretreated with cecropin A; cecropin A + E. coli: cells were pretreated with cecropin A and then cocultured with E. coli; E. coli: cells cocultured with E. coli. The results were confirmed by three independent experiments per treatment. Representative results of the three independent experiments are shown. Data (mean ± SEM) were analyzed with one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001.