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. 2018 Jul 4;10(7):863. doi: 10.3390/nu10070863

Figure 3.

Figure 3

Effect of kahweol on lipopolysaccharide (LPS)-induced expression and production of IL1α, IL1β, IL6, and TNFα in primary Kupffer cell (KC) and primary hepatocyte (HC) co-culture. (A) Representative real-time RT-PCR analysis of IL1α, IL1β, IL6, and TNFα mRNA expression in co-culture of primary KC with primary HC. Data in the bar graph are mean ± SEM of three independent measurements. ** p < 0.01 compared with control (Con), # p < 0.05, ## p < 0.01 compared with LPS. (B) Primary KC and primary HC co-cultures media were collected after LPS and kahweol (kah) treatment, the production of IL1α, IL1β, IL6, and TNFα were measured using the ELISA kits. Data in the bar graph are mean ± SEM of three independent measurements. * p < 0.05, ** p < 0.01 compared with control (Con), # p < 0.05, ## p < 0.01 compared with LPS.