Figure 6.

dsDNA-damaging effects of irradiation (IR) on pADSCs and their repair capacity within 24 h. Phosphorylated H2AX (γH2AX) was used as a marker for DNA double-strand breaks (DSBs). To determine γH2AX foci, cells were fixed 1 h or 24 h after IR and incubated with anti-γH2AX antibody and IgG1 (red); DNA counterstaining with 4,6-diamidino-2-phenylindole (DAPI) (blue). (A) Exemplary images of immunocytochemistry staining at different time points after IR. On the left corner of each image, results are illustrated as mean ± standard deviation (SD, n = 3); (B) Graphical illustration of mean number of γH2AX foci per cell; asterisks illustrate significant differences to unirradiated cells (control): * p < 0.05; ** p < 0.01; *** p < 0.001.