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. 2018 Jul 20;19(7):2115. doi: 10.3390/ijms19072115

Figure 4.

Figure 4

Effect of cN-II silencing on enzyme activities, mitochondrial ROS, mass and membrane potential in A549 cells. (A) LDH, (B) hexokinase and (C) CS were assayed as described in Materials and Methods in cell extracts obtained from cultured pScont and pScNII incubated 24 h with or without 20 mM dG. Each experiment was performed in triplicate and repeated at least three times; (D) mitochondrial ROS; (E) mitochondrial mass; (F) mitochondrial potential. The analysis was performed with confocal microscopy as described in Material and Methods in pScont and pScNII cells incubated for 24 h in the presence or absence of 20 mM dG. Data are the average + SEM of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.