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. 2018 Jul 20;19(7):2115. doi: 10.3390/ijms19072115

Figure 6.

Figure 6

Effect of cN-II silencing on protein synthesis in A549 cells. Newly synthetized proteins were labeled using the Click-iT® HPG Alexa Fluor Protein Synthesis kit (see Methods for details). The incorporation of the methionine analogue HPG was monitored with confocal microscopy. Left: optical density (OD) of the fluorescence signal generated by Alexa Fluor 488-azide crosslinked to HPG in A549 cells pScont and pScNII with or without 20 mM dG. Results are the mean + SEM of three independent experiments. * p < 0.05, *** p < 0.001, **** p < 0.0001. Right: confocal images of A549 cells after HPG incorporation and Alexa Fluor 488-azide reaction. Calibration bar = 30 µm applies to all micrographs.