Table 1.
Substrate specificity of LaOMT1.
| Substrate | Product | Km (μM) | Kcat (min−1) | Kcat/Km (min−1 mM−1) | |
|---|---|---|---|---|---|
| caffeic acid | ferulic acid | isoferulic acid | 20.32 ± 4.81 | 0.023 ± 0.001 | 1.12 |
| 3,4-dihydroxybenzaldehyde | vanillin | isovanillin | 151.94 ± 23.32 | 1.04 ± 0.04 | 6.83 |
| norbelladine | 3′-O-methylnorbelladine | 4′-O-methylnorbelladine | - | - | - |
| vanillin | ND | ND | ND | ND | ND |
| isovanillin | ND | ND | ND | ND | ND |
| o-vanillin | ND | ND | ND | ND | ND |
| tyramine | ND | ND | ND | ND | ND |
ND stands for “not detectable”. - represents product made by LaOMT1 but not quantified.