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The hnRNP K phosphorylation status correlates with AR activity in prostate cancer cell lines. Nuclear (a) and cytoplasmic (b) profiles of hnRNP K phosphorylated isoforms in androgen-responsive LNCaP and resistant cell lines PDB, MDB, 22Rv1, and PC3, evaluated using 2D Phos-tag and WB analysis. The membranes were probed with an anti-hnRNP K antibody. The phK0, phK1, and phK2 identified in Phos-tag 1D (Figure 2b) are indicated.
